EIF2AK4 Knockout cell line (L-02)

ENCN
location： Home > Products > KO Cell Lines > EIF2AK4 Knockout cell line (L-02)

EIF2AK4 Knockout cell line (L-02) (Hela-contaminated cells, not for sales) Order

EIF2AK4 Knockout cell line (L-02)
Catalog#
YKO-H845
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
EIF2AK4 Knockout cell line (L-02)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
EIF2AK4 Knockout cell line (L-02)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
EIF2AK4
Gene ID
440275
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
80% RPMI-1640+20% FBS
Accession
CVCL_E0X4(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 4 (based on transcript EIF2AK4-201)
Validation: PCR+Sanger Sequencing
EIF2AK4 Knockout cell line (L-02) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:L-02		 STR Info (Cell bank)
Cell Line:L-02
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 13.313.313.313.3
D7S820 12121212
D16S539 910910
VWA 16161618
TH01 7777
AMEL XXXX
TPOX 12121212
CSF1PO 10101010
D12S391 2020
FGA 1821
D2S1338 1717
D21S11 2728
D18S51 1616
D8S1179 1212
D3S1358 1518
D6S1043 1818
PENTAE 717
D19S433 1313
PENTAD 815
D1S1656 1215
* Hela-contaminated cells, not for sales
Reviewed by ICLAC (ref: 190616). L-02 was reported to be established from normal liver cells but its STR profile corresponds to HeLa. Samples tested in this study were directly deposited in the cell line repository that performed the investigation. L-02 has a derivative: HL-7702BaPT. No STR profiling information was available for the derivative at the time of review.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the EIF2AK4 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the L-02 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, EIF2AK4 gene knockout EIF2AK4 Knockout cell line (L-02) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
EIF2AK4 Knockout cell line (L-02)
EIF2AK4 Knockout cell line (L-02)
Catalog#
YKO-H845
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

  • L-02 cell line

    Human Normal Hepatocytes Cell line(L-02)
    Hela-contaminated cells, not for sales
      inquiry   More details

    Catalog#: YC-D004
    Size: 1*10^6
    Instruction: Human Normal Hepatocytes Cell line(L-02)

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • EIF2AK4 Knockout cell line (HeLa)

    EIF2AK4 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: EIF2AK4
    Gene ID: 440275
    Size: 1*10^6
    Catalog#: YKO-HS08184
    YKO-HS08184(HeLa)
  • EIF2AK4 Knockout cell line (HCT 116)

    EIF2AK4 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: EIF2AK4
    Gene ID: 440275
    Size: 1*10^6
    Catalog#: YKO-HS08183
    YKO-HS08183(HCT 116)
  • EIF2AK4 Knockout cell line (HEK293)

    EIF2AK4 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: EIF2AK4
    Gene ID: 440275
    Size: 1*10^6
    Catalog#: YKO-HS08182
    YKO-HS08182(HEK293)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • EIF2AK4 Knockout cell line (HeLa)

    EIF2AK4 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: EIF2AK4
    Gene ID: 440275
    Size: 1*10^6
    Catalog#: YKO-HS08184
      inquiry   More details
  • EIF2AK4 Knockout cell line (HCT 116)

    EIF2AK4 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: EIF2AK4
    Gene ID: 440275
    Size: 1*10^6
    Catalog#: YKO-HS08183
      inquiry   More details
  • EIF2AK4 Knockout cell line (HEK293)

    EIF2AK4 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: EIF2AK4
    Gene ID: 440275
    Size: 1*10^6
    Catalog#: YKO-HS08182
      inquiry   More details
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
×

Search documentation

Literature:

Name: *

Company: *

Telephone: *

Email:

Notes: After submitting the order, we will contact you as soon as possible.

request now